Journal: Pathology and Oncology Research

Article Title: 3D bioprinting and the revolution in experimental cancer model systems—A review of developing new models and experiences with in vitro 3D bioprinted breast cancer tissue-mimetic structures

doi: 10.3389/pore.2023.1610996

Figure Lengend Snippet: Bioinks in cancer research.

Article Snippet: , Hyaluronic-acid , Tumour spheroids , Excellent biocompatibility , Poor mechanical strength , Physical or covalent , Cellink - PhotoHA®, Advanced Biomatrix - PhotoHA® , ( , ) .

Techniques: Derivative Assay, Immunopeptidomics, Viscosity, Control, Solubility, In Vivo, Concentration Assay, Construct

Journal: Journal of Dental Research

Article Title: Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

doi: 10.1177/0022034514540682

Figure Lengend Snippet: Summary of Biomatrices Currently Available and Recently Generated by Advanced Approaches

Article Snippet: Biofactor-delivering Biomatrices While the immobile ECM molecules are the first design criterion producing biomimetic scaffolds, a cocktail of soluble bioactive molecules, such as growth factors and cytokines, should also be considered as critical in the regeneration of periodontal tissues.

Techniques: Generated, Clinical Proteomics

Journal: Journal of Dental Research

Article Title: Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

doi: 10.1177/0022034514540682

Figure Lengend Snippet: Biomatrices designed to mimic the native extracellular matrix (ECM) compositions. (A) Bone mineral apatite. (A-a) Two-dimensional cryo-TEM image. (A-b) Slice from a section of the three-dimensional volume. (A-c) Computer-generated three-dimensional visualization of mineralized collagen (scale bar, 100 nm). Cryo-TEM images of (A-d) stained, non-mineralized collagen, (A-e) mineralized collagen, and (A-f) stained fibril (mineralized) (scale bar, 50 nm). (A-g) Intensity profile of A-d; (A-h) intensity profile of A-e; and (A-i) histogram of the distribution of the number of nucleating crystals per staining band (Nudelman et al., 2010). (B) Hydroxyapatite (HA)-collagen (COL) nanocomposites: SEM images of the HA scaffold coated (B-a) with non-cross-linked COL with compact COL; (B-b) collagen fibers; (B-c) with EDC/NHS cross-linked COL; and (B-d) heterogeneous COL distribution. (B-e) BMP-2 cumulative release profiles: HA scaffold (♦), HA scaffold coated with COL (■), and HA scaffold coated with COL/heparin conjugate (▲). (B-f) Concentration of BMP-2 retained: HA, HA scaffold with COL (HA XL COL) and HA scaffold with COL and heparin (HA COL HEP). Scale bars: 600 µm for B-a and B-c; 30 µm for B-b and B-d (Teixeira et al., 2010).

Article Snippet: Biofactor-delivering Biomatrices While the immobile ECM molecules are the first design criterion producing biomimetic scaffolds, a cocktail of soluble bioactive molecules, such as growth factors and cytokines, should also be considered as critical in the regeneration of periodontal tissues.

Techniques: Generated, Staining, Concentration Assay

Journal: Journal of Dental Research

Article Title: Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

doi: 10.1177/0022034514540682

Figure Lengend Snippet: Biomatrices designed to mimic the native extracellular matrix (ECM) micro/nano-structures. (A) Nanofibrous scaffolds. (A-a) SEM of nanospheres (scale bar, 500 nm); (A-b) macroscopic photographs of scaffolds (scale bar, 1 mm); SEM of nano-fibrous scaffolds before nanosphere incorporation at 100x (A-c) and 10,000x (A-d); and SEM of nano-fibrous scaffolds after nanosphere incorporation at 100x (A-e) and 10,000x (A-f). Scale bars: 200 µm for A-c and A-e; 2 µm for A-d and A-f (Wei et al., 2007). (B) 3D printed scaffolds to mimic periodontal ligament (PDL) microscopic structure. (B-a) Micro-computed tomographic image of mandibular defect; (B-b) computer-designed scaffold; (B-c, B-d, B-e) computational adaptation of scaffold; (B-f, B-g) freshly sectioned images of the scaffold; and (B-h) type-I collagen fluorescence (yellow arrows: type-I collagenous fiber bundles). Scale bar: 50 µm (Park et al., 2014).

Article Snippet: Biofactor-delivering Biomatrices While the immobile ECM molecules are the first design criterion producing biomimetic scaffolds, a cocktail of soluble bioactive molecules, such as growth factors and cytokines, should also be considered as critical in the regeneration of periodontal tissues.

Techniques: Fluorescence

Journal: Journal of Dental Research

Article Title: Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

doi: 10.1177/0022034514540682

Figure Lengend Snippet: Biomatrices driven by cells. (A) Demineralized freeze-dried bone matrix (DFDBM). (A-a, A-b) SEM images of DFDBM; (A-c, A-d) blood-coated DFDBM particles; and (A-e, A-f) enamel matrix derivative (EMD)-coated DFDBM particles. Scale bars: 100 µm for A-a, A-c, and A-e; 50 µm for A-b, A-d, and A-f (Miron et al., 2013). (B) Cell-sheet engineering. (B-a) Schematic illustration of periodontal defects and three-layered cell-sheet transplantation. (B-b) Surgically created intrabony defects. (B-c) Transplantations of three-layered periodontal ligament (PDL) cell sheets and β-TCP. (B-d, B-e, B-f, B-g) Micro-CT analysis of periodontal defects after six-week period of healing. Photomicrographs of contralateral defect sites receiving β-TCP (B-h) and PCL cell sheet/β-TCP (B-i). Polarized light-microscopic images (B-j, B-k). Scale bar: 500 µm (Iwata et al., 2009).

Article Snippet: Biofactor-delivering Biomatrices While the immobile ECM molecules are the first design criterion producing biomimetic scaffolds, a cocktail of soluble bioactive molecules, such as growth factors and cytokines, should also be considered as critical in the regeneration of periodontal tissues.

Techniques: Transplantation Assay, Micro-CT

Journal: Journal of Dental Research

Article Title: Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

doi: 10.1177/0022034514540682

Figure Lengend Snippet: Designs of therapeutic biomatrices to load and deliver bioactive molecules.

Article Snippet: Biofactor-delivering Biomatrices While the immobile ECM molecules are the first design criterion producing biomimetic scaffolds, a cocktail of soluble bioactive molecules, such as growth factors and cytokines, should also be considered as critical in the regeneration of periodontal tissues.

Techniques:

Journal: Pathology and Oncology Research

Article Title: 3D bioprinting and the revolution in experimental cancer model systems—A review of developing new models and experiences with in vitro 3D bioprinted breast cancer tissue-mimetic structures

doi: 10.3389/pore.2023.1610996

Figure Lengend Snippet: Bioinks in cancer research.

Article Snippet: , Pluronic , Vascularised tissues, complicated tissue constructs , High shape fidelity , Short of cell-binding domains , Covalent , Cellink - Pluronics 40%, Advanced Biomatrix - Pluronics 40% , ( ) .

Techniques: Derivative Assay, Immunopeptidomics, Viscosity, Control, Solubility, In Vivo, Concentration Assay, Construct

Journal: bioRxiv

Article Title: Organoid-based colorectal tumor microenvironment model for immuno-oncology research

doi: 10.64898/2026.06.02.729459

Figure Lengend Snippet: (A) Multiplex immunofluorescence images of SHOCOs at day 5 of culture from three different patients (P464, P634, P599) show polarization of the tumor epithelium (grey), indicated by the expression of CEACAM5 (green) towards the lumen. (B) Brightfield images of SHOCOs seeded in different hydrogels (either reconstituted basement membrane or Collagen I at Day 0, Day 1, and Day 6. Scale bars: white 100µm, black 1mm.

Article Snippet: The cell suspension containing all three populations was then centrifuged (3 min, 300 g), the supernatant was completely removed without disturbing the pellet, and finally, the cells were resuspended in 2 mg/ml Collagen I (5225-50ML, BIOMATRIX) and kept on ice until needed.

Techniques: Multiplex Assay, Immunofluorescence, Expressing, Membrane